August 3, 2020

How to successfully sterilise laboratory glasswares and equipment?

How to successfully sterilise laboratory glasswares and equipment?

In most scientific laboratory, there are many applications that require sterilie equipment.  These equipment ranging from a normal laboratory glasswars to medical grade appliances.   The wide variety of these equipment creates a challenge to successfully achive complete sterilisation.

There are a number of considerations that need to be taken into account.

  • Most obviously, avoiding glass breakage or shattering within the chamber.
  • Empty glassware should never be sealed where high vacuum is used to dry sterilized glassware.
  • Care should be taken when loading and unloading to avoid damage, overcrowding or scalding.
  • Drying of glassware loads to remove condensate can be accommodated (if required).
  • All types of instrument loads benefit from drying at the end of the cycle.
  • Unwrapped instruments can be dried by the heating of the surrounding air at the end of the cycle.
  • Post vacuum systems on small capacity bench-top units will improve drying, whilst a jacketed pressure vessel is strongly recommended on larger capacity units.

Based on these challenges, there are few options that needs to be considered when choosing an autoclave to handle this type of load.

  • Baskets And Morrison Containers – available in various shapes and sizes to ensure glassware is safely contained for easy loading and
    unloading from the autoclave.
  • Advanced Vacuum (AVC001) – will significantly improve steam penetration and effective air removal, and with the addition of a
    Heated Jacket will also assist with the removal of condensate at the end of the cycle should dry loads be required.
  • Basic Vacuum System (AVC002) – is only applicable to standard autoclaves with heaters in the chamber, or direct steam models. It isideal for situations where improved air removal is required, such as for vacuum cooling of unwrapped items.

Related Products

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The 125 – 735 litre SQUARE autoclave range is easily customizable and with a range of different chamber sizes. The SQUARE range is Astell’s most popular range of square chambered autoclaves, capable of sterilizing almost anything (additional options may be required).

Top Loading Autoclaves

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The 95 – 135 litre toploading autoclave range offers increased chamber sizes compared to the ‘Compact’ units, as well as featuring advanced options not available on smaller sized autoclaves, including Heated Jackets, Integral Steam Generators, Simple/Advanced Vacuum and Cooling options.

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The 250 - 1,200 litre Double Door pass-through SQUARE Autoclave Range is ideal for high-containment type (CL-3 / BSL-3) facilities or where a specific area or 'clean room' are in operation, as the sterilized load can 'pass through' the autoclave to be removed in to this environment.