Cowside II

Charm Cowside II Test is a broad-spectrum inhibition test typically used for screening of raw commingled and ultra-pasteurized cow milk on the farm.

It detects all major classes of antibiotics: beta-lactams, sulfonamides, tetracyclines, macrolides, and aminoglycosides. Eleven drugs are detected at or below US Safe Levels and 30 drugs at or below EU MRLs.

Cowside II

Each kit contains materials for 20 or 100 tests. Add sample, incubate, and then compare the vial color to reference colors for the result.

Cowside II Test kits consist of single service wells that contain pre-measured bacterial spores, media, and a pH indicator. The starting color in the well is blue/purple. Milk is added to the well and incubated. If antibiotics are absent, spores germinate and grow, which changes the color to yellow or yellow/green. If antibiotics are present in the milk, microbial growth is inhibited and the positive sample remains blue/purple.

Benefits

  • Detects all major classes of antibiotics: beta-lactams, sulfonamides, tetracyclines, macrolides, and aminoglycosides
  • Detects 11 drugs at or below US Safe Levels
  • Detects 30 drugs at or below EU MRLs
  • Approved by the New Zealand Food Safety Authority (NZFSA)
  • Ideal for large volume testing
  • Easy: Just add milk and incubate
  • Results in about three hours
  • Results read visually

Regulatory Info

Charm Cowside II detects 11 drugs at or below US safe levels.

It has not been evaluated by National Conference on Interstate Milk Shipments (NCIMS).

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Charm Cowside II detects 24 drugs at or below EU MRLs.

Central Reference Laboratory (CRL) guidelines available upon request.

Approvals/Validations:

Approved by New Zealand Food Safety Authority (NZFSA)

Approved by Polish Veterinary Research Institute (PIWET)

Approved by Chief Veterinary Inspectorate (Warsaw, Poland)

Approved by State Committee for Veterinary Medicine of Ukraine

Sensitivity

 
Antibiotic Cowside Sensitivity (ppb) US Safe Level (ppb) EU/ CODEX MRL (ppb)
Amoxicillin 3-4 10 4/ 4
Ampicillin 3-4 10 4
Cefacetrile 10-15 None 125
Cefalexin 75-100 None 100
Cefalonium 15-20 None 20
Cefazolin 6-10 None 50
Cefoperazone 20-30 None 50
Cefquinome 40-60 None 20
Ceftiofur and Metabolites 50-100 100 100/ 100
Cefuroxime 20-25 None None
Cephapirin 8-10 20 60
Chlortetracycline 200-300 300 100/100
Cloxacillin 10-25 10 30
Dapsone 1-2 None 0
Dicloxacillin 5-10 None 30
Doxycycline 25-75 None 0
Erythromycin 75-100 50 40
Gentamicin 75-150 30 100/ 200
Nafcillin 5-10 None 30
Neomycin 100-150 150 1500/ 1500
Oxacillin 5-10 None 30
Oxytetracycline 75-100 300 100/ 100
Penicillin G 2-3 5 4/ 4
Pirlimycin 25-50 400 100/ 100
Spiramycin 300-400 None 200/ 200
Sulfadiazine 40-60 10 100
Sulfadimethoxine 25-50 10 100
Sulfamethazine (Sulfadimidine) 75-125 10 100/ 25
Tetracycline 50-100 300 100/ 100
Tilmicosin 25-35 None 50
Trimethoprim 200-300 None 50
Tylosin 20-30 50 50/ 100